Figure 4.
Expression and production of SMOX in TR-MUL5 cells with silencing of Hif1a or Hif2a under hypoxic condition (1% O2). TR-MUL5 cells were transfected with control siRNA (Ctrl-siRNA), siRNAs for Hif-1α (Hif1a siRNA-1 and -2), or siRNAs for Hif2a (Hif2a siRNA-1 and -2) and cultivated with or without hypoxic stimulation. (A) Real-time qPCR analysis for Smox mRNA expression 6 hours after hypoxic stimulation. (B) SMOX protein levels in TR-MUL5 cells measured by ELISA 24 hours after hypoxia stimulation. (C) Cell viability assay. n = 3 in each group. **P < 0.01, n.s., not significant.