Scheme 2.

Workflow of the reactions from each condition to determine the effect of H₂O₂ added after the flavin reduction and formation of imino acid. After the flavin reductions, the active enzyme reactions were added into aerobic buffer (E1), anaerobic buffer (E2), formic acid to denature the enzyme and H₂O₂ in aerobic buffer (E3), H₂O₂ in anaerobic buffer (E4), and H₂O₂ in aerobic buffer (E5). The effect of H₂O₂ preincubated with the enzyme before flavin reduction was carried out under all anaerobic conditions (E6).