Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 29;295(32):10926–10939. doi: 10.1074/jbc.RA120.014103

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Darlot et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

PMC Copyright notice

Figure 2. — Design and biophysical analysis of an EVA1/P672 hybrid protein. A, alignment of EVA1, P672, and EVA1/P672 (EVA1 containing P672_E22–E32) hybrid protein using MUSCLE algorithm. Amino acids are color-coded according to physicochemical properties: yellow, aromatic (Phe, Trp, and Try); red, acidic (Asp and Glu); blue, basic (Arg, His, and Lys); orange, nonpolar aliphatic (Ala, Gly, Ile, Leu, Met, Pro, and Val); and green, polar neutral (Cys, Asn, Gln, and Thr). Amino acids that were protected from deuterium uptake in P672 are indicated with a red box. The N-terminal acidic region is enclosed in a black box. B, biolayer interferometry sensorgram obtained when either P672, EVA1/P672, or EVA1 is loaded onto the biolayer interferometry sensor and exposed to 600 nm CCL8. Plots display wavelength shift (y axis, nm) versus time (x axis, s). C, biolayer interferometry sensorgram for EVA1/P672 hybrid binding to CCL8. Dotted lines indicate collected data, and solid lines indicate modeled data. Plots display wavelength shift (y axis, nm) versus time (x axis, s).