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. 2020 Jun 16;295(32):11161–11173. doi: 10.1074/jbc.RA120.013618

Figure 2.

Figure 2.

The kindlin-3 C-terminal subdomains are central to self-association. A, association of GFP or GFP-kindlin-3 (GFP-K3) with FLAG-kindlin was assessed by GFP pulldown and immunoblotting and compared with 5% input. B, binding was quantified by normalization to GFP-kindlin-3 + FLAG-kindlin-3 (GFP-K3+FLAG-K3) association; mean ± S.E.; n = 3; *, p ≤ 0.0001 (Student's t test). C, cartoon schematic of kindlin FERM domain architecture and kindlin chimeras. D and E, association of GFP or GFP-kindlin-3 (GFP-K3) with FLAG-kindlin-3 (FLAG-K3) or FLAG-kindlin chimeras was assessed by GFP pulldown (D) and quantified by normalization to GFP-kindlin-3 + FLAG-kindlin-3 association (GFP-K3+FLAG-K3) (E); mean ± S.E.; n ≥ 3; *, p ≤ 0.007 (Student's t test).