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. 2020 Jun 16;295(32):11161–11173. doi: 10.1074/jbc.RA120.013618

Figure 3.

Figure 3.

The F2PH subdomain is important for kindlin-3 self-association. A, association of GFP-kindlin-3 (GFP-K3) with FLAG-kindlin chimeras was assessed by GFP pulldown. 5% input was loaded for comparison. B, binding was quantified by normalization to GFP-kindlin-3 + FLAG-kindlin-3 association; mean ± S.E.; n ≥ 3; *, p ≤ 0.009 (Student's t test). C, association of GFP-kindlin-3 (GFP-K3) with FLAG-kindlin was assessed by GFP pulldown. Input lane is 5%. D, binding was quantified by normalization to GFP-kindlin-3 + FLAG-kindlin-3 association (GFP-K3+FLAG-K3); mean ± S.E.; n ≥ 3; *, p ≤ 0.001 (Student's t test).