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. 2020 Jun 16;295(32):11161–11173. doi: 10.1074/jbc.RA120.013618

Figure 6.

Figure 6.

A kindlin-3 mutant with enhanced integrin binding is impaired in self-association. A and B, association of GFP-kindlin-3 (GFP-K3) or GFP-kindlin-3 F3mutx3 (GFP-K3 F3mutx3) with FLAG-kindlin was assessed by GFP pulldown and immunoblotting (A) and quantified by normalization to GFP-kindlin-3 + FLAG-kindlin-3 self-association (GFP-K3+FLAG-K3) (B); mean ± S.E.; n = 6. *, p ≤ 0.03 (Student's t test). C, association of GFP-kindlin-2 (GFP-K2) or GFP-kindlin-2 F3mutx3 (GFP-K2 F3mutx3) with FLAG-kindlin was assessed and compared with a 5% input. D, binding was quantified and normalized to GFP-kindlin-3 + FLAG-kindlin-3 self-association (GFP-K3+FLAG-K3); mean ± S.E.; n = 3; ns, not statistically significant, p > 0.05.