Table 1.

Plasmid complementation test

Plasmids were introduced into ME5491 (dnaB43) cells. Transformants were incubated at 30 °C for 19 h or 42 °C for 14 h on LB-agar plates containing 0.1% arabinose and 50 µg/ml ampicillin, and then colonies were counted. Transformation efficiency (colony-forming units/µg of DNA) at each temperature and ratios of efficiencies are shown. Efficiency of pBAD/His-DnaB was defined as 1. ^a, only tiny colonies with a diameter of <0.5 mm were formed.

Plasmid	dnaB allele	Transformation efficiency (× 105 transformants/μg of DNA)		Ratio (42 °C/30 °C)
		30 °C	42 °C
pBAD18 (vector)	None	2.0	<1.0 × 10−2	<7.4 × 10−3
pBAD/His-DnaB	WT	8.9	6.1	[1.0]
pMS17	L158A	5.9	<1.0 × 10−2	<2.5 × 10−3
pMS02	L160A	9.4	2.1a	0.33
pMS03	R164A	6.4	2.7a	0.62
pMS18	V165A	9.3	<1.0 × 10−2	<1.6 × 10−3
pMS04	K167A	14	7.6	0.80
pMS19	I168A	8.2	<1.0 × 10−2	<1.8 × 10−3
pMS10	R172A	2.0	0.20	0.15
pMS11	K175A	2.2	0.64	0.43
pMS12	P179A	2.6	1.1	0.62
pMS07	V190A	7.4	7.4a	0.57
pMS13	P200A	2.0	0.76	0.56
pMS14	H201A	2.6	0.21	0.12
pMS15	V204A	2.4	0.56	0.34

^a Tiny colonies (<0.5 mm).