Figure 4.

The proinflammatory responses in H. pylori-infected EDMs derived from murine gastric tissue depend on NEIL2. A, the schematic diagram shows the plan for functional assays of enteroids isolated from WT or Neil2 KO mice. B, the proteome profiler array was conducted with the supernatant collected from the H. pylori-infected WT and Neil2 KO EDMs. Two blots from low exposures and high exposures were analyzed, and the cytokines that appeared in the array are listed in the table. C, the differential expression of selected cytokines was verified by qRT-PCR of RNA samples isolated from EDMs used in panel B. D–F, the supernatants from the EDMs of WT or Neil2 KO mice, either untreated (Un) or infected with H. pylori (Hp), were further verified by ELISA for KC, MCP-1, and IL-6 expression. Data were generated from the mean ± S.D. from three independent experiments. *, p < 0.05; **, p < 0.01.