Table 2.
List of primers used in the study
| Gene | Species | Forward primer (5′→3′) | Reverse primer (5′→3′) |
|---|---|---|---|
| NEIL2 | Mouse | CTGCCGCCTTTCAGTCTCT | TCTGGATCAAACCGAAGGAA |
| Human | GGGGCAGCAGTAAGAAGCTA | GGAATAATTTCTTTCCATGGACCT | |
| IL-8/KC | Mouse | CGCTTCTCTGTGCAGCGCTGCTGCT | AAGCCTCGCGACCATTCTTGAGTC |
| Human | GAGCACTCCATAAGGCACAAA | ATGGTTCCTTCCGGTGGT | |
| IL-6 | Mouse | CCCCAATTTCCAATGCTCTCC | CGCACTAGGTTTGCCGAGTA |
| Human | TCAATATTAGAGTCTCAACCCCCA | TTCTCTTTCGTTCCCGGTGG | |
| IFN-γ | Mouse | CGGCACAGTCATTGAAAGCC | TGTCACCATCCTTTTGCCAGT |
| Human | GGCTTTTCAGCTCTGCATCG | TCTGTCACTCTCCTCTTTCCA | |
| MCP-1 | Mouse | AAGTGCAGAGAGCCAGACG | TCAGTGAGAGTTGGCTGGTG |
| Human | AGTCTCTGCCGCCCTTCT | GTGACTGGGGCATTGATTG | |
| TNF-α | Mouse | CCACCACGCTCTTCTGTCTA | AGGGTCTGGGCCATAGAACT |
| Human | CGCTCCCCAAGAAGACAG | AGAGGCTGAGGAACAAGCAC | |
| IL-1β | Mouse | GAAATGCCACCTTTTGACAGT | CTGGATGCTCTCATCAGGACA |
| Β-actin | Mouse | GACGGCCAGGTCATCACTAT | ACATCTGCTGGAAGGTGGAC |
| Human | AGAGCTACGAGCTGCCTGAC | AGCACTGTGTTGGCGTACAG | |
| NEIL2 FLAG | ATGCCAGAAGGGCCGTTG | TTGTCATCGTCGTCCTTGTAGT | |
| Endogenous NEIL-2 | ATGCCAGAAGGGCCGTTG | GGAGAACTGGCACTGCTCTG |