Figure 4.

Interferon‐γ (IFN‐γ) induced the α2AP production through the c‐Jun N‐terminal kinase (JNK) pathway in fibroblasts. A, NIH3T3 cells were stimulated by IFN‐γ (10, 20 ng/mL) for 24 hours. The expression of α2AP was measured by Western blot analysis. The histogram on the bottom panels shows quantitative representations of α2AP expression obtained from densitometry analysis (n = 3). B, NIH3T3 cells were cultured for 24 hours in the absence or presence of IFN‐γ (20 ng/mL), PD98059 (30 μM), or SP600125 (30 μM) as indicated. The histogram on the bottom panels shows quantitative representations of α2AP expression obtained from densitometry analysis (n = 3). C, NIH3T3 cells were stimulated by IFN‐γ (20 ng/mL) for the indicated periods. The expression of each protein was measured by Western blot analysis. The histogram on the bottom panels shows quantitative representations of phospho‐JNK expression obtained from densitometry analysis (n = 3). The data represent the mean ± SEM. *P < .01. **P < .05. α2AP, alpha2‐antiplasmin; NS, not significant