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Effect of prime/boost vaccination on gene expression in the lungs upon infection. Cotton rats were (mock‐)immunized, boosted and challenged as previously described. On day 1 and day 10 after virus challenge, expression of cytokine mRNA in lungs of the animals was assessed by qRT‐PCR with gene‐specific primers using the housekeeping gene GAPDH as a reference. GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; mRNA, messenger RNA; qRT‐PCR, quantitative real‐time polymerase chain reaction
