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. 2020 Aug;22(8):1050–1062. doi: 10.1016/j.jmoldx.2020.05.005

Table 1.

Capture and Detection Oligonucleotides for eLB EGFR Assay

Variant Probes
Capture Probes
Exon 19del 5′-TGTTGCTTCCTTG-3′
p.L858R 5′-GTTTGACCCGCCCA-3′
p.T790M 5′-GAGCGGCATGATGA-3′
Detector Probes
Exon 19del 5′-ATAGCGACGGGAATTTTAACTTTCTCACCT-3′
p.L858R 5′-AAAATCTGTGATCTTGACATGCTGCGGTGTTTTGTGCAG-3′
p.T790M 5′-GCTGCACGGTGGAGGTGAGGCAGATGCCCAGC-3′

eLB, electric field–induced release and measurement liquid biopsy.

Detector probes are biotin labeled at the terminal 3′ nucleotide.