Table 2. Main fatty acids (FA, expressed as mass fraction of total identified fatty acids) of the total, neutral and polar lipids and free fatty acids in Sheffersomyces stipitis wild type and M12 yeast strain and the main features of the composition.
| Fatty acid | w(FA)/% | |||||||
|---|---|---|---|---|---|---|---|---|
| Total lipids | Neutral lipids | Polar lipids | Free fatty acids | |||||
| Wild type | M12 strain | Wild type | M12 strain | Wild type | M12 strain | Wild type | M12 strain | |
| C 15:0 | n.d. | n.d. | 2.6±0.6 | 1.9±0.6 | n.d. | (3.1±1.0)* | 4.9±0.5 | (0.03±0.05)* |
| C 16:0 | 14.5±0.4 | 13.6±0.5 | 20.9±2.9 | (16.0±0.6)* | 10.8±0.3 | (17.3±1.2)* | 45.4±2.5 | (22.0±1.4)* |
| C 16:1 | 3.0±0.3 | (2.4±0.1)* | 2.4±0.3 | 1.6±0.7 | 3.0±0.3 | (2.3±0.3)* | 6.5±0.4 | (5.0±0.7)* |
| C 18:0 | 3.0±0.5 | (5.4±0.2)* | 5.0±0.4 | (7.7±0.9)* | 2.1±0.3 | (4.4±0.5)* | 9.2±1.4 | (5.0±0.9)* |
| C 18:1 | 27.5±0.8 | (38.4±1.1)* | 26.6±2.2 | (44.4±1.6)* | 25.4±0.4 | (31.4±0.7)* | 30.5±3.5 | (40.4±1.6)* |
| C 18:2 | 48.3±1.1 | (37.0±1.1)* | 38.6±2.6 | (25.7±2.1)* | 53.2±1.1 | (32.1±1.7)* | 2.1±0.5 | (25.9±0.4)* |
| C 18:3 | 3.2±0.2 | (2.8±0.1)* | 1.9±0.6 | (1.1±0.3)* | 4.7±0.1 | (2.1±0.3)* | 0.1±0.1 | (1.4±0.3)* |
| C 20:1 | 0.23±0.05 | 0.21±0.02 | 1.1±0.4 | 0.6±0.2 | 1.1±0.4 | 0.6±0.2 | 0.8±0.0 | n.d.* |
| c 22:3 | 0.08±0.03 | 0.08±0.03 | n.d. | (0.4±0.2)* | 0.3±0.1 | (3.2±0.9)* | n.d. | n.d. |
| c 22:4 | 0.10±0.02 | 0.09±0.02 | 0.7±0.1 | 0.6±0.2 | 0.4±0.1 | (3.5±1.1)* | 0.4±0.2 | n.d.* |
| UFA | 82.4±0.7 | (81.0±0.3)* | 71.3±4.0 | 74.5±1.7 | 88.1±0.7 | (75.2±1.0)* | 40.4±0.6 | (72.7±0.8)* |
| mono- | 30.7±0.3 | (41.0±0.2)* | 30.1±2.2 | (46.6±1.6)* | 29.5±0.2 | (34.3±0.6)* | 37.9±0.2 | (45.4±0.6)* |
| poli- | 51.7±0.9 | (40.0±0.8)* | 41.2±2.6 | (27.8±2.1)* | 58.6±1.0 | (40.9±1.2)* | 2.6±0.3 | (27.3±1.2)* |
| C16 | 17.5±0.7 | (16.00±0.5)* | 23.3±0.7 | (17.6±0.5)* | 13.8±0.3 | (19.6±1.6)* | 51.9±0.2 | (27.0±1.4)* |
| C18 | 82.0±0.6 | (83.6±0.5)* | 72.1±2.1 | (78.9±0.6)* | 85.4±0.6 | (70.0±0.4)* | 42.0±0.7 | (72.7±1.3)* |
| SFA/UFA | 0.21±0.01 | (0.24±0.01)* | 0.41±0.09 | 0.35±0.03 | 0.15±0.01 | (0.33±0.02)* | 1.47±0.03 | (0.43±0.01)* |
| IU | 1.38±0.02 | (1.24±0.02)* | 1.17±0.09 | (1.06±0.01)* | 1.52±0.01 | (1.35±0.03)* | 0.44±0.01 | (1.02±0.03)* |
Mean value±S.D. of at least three independent experiments performed in duplicate was calculated on the basis of peak areas; *significantly different from the wild type (Mann-Whitney U test, p<0.05), n.d.=not detected