Table 3. Main fatty acids (FA, expressed as mass fraction of total identified fatty acids) of the triacylglycerols and sterol esters in Sheffersomyces stipitis wild type and M12 strain and the main features of the composition.
Fatty acid | w(FA)/% | |||
---|---|---|---|---|
Triacylglycerols | Sterol esters | |||
Wild type | M12 strain | Wild type | M12 strain | |
C 16:0 | 14.3±1.0 | 13.7±0.8 | 7.1±0.8 | (11.4±1.1)* |
C 16:1 | 2.2±0.2 | (1.7±0.2)* | 3.3±0.2 | (2.70±0.05)* |
C 18:0 | 8.0±0.6 | (11.2±1.4)* | 1.6±0.2 | 1.8±0.6 |
C 18:1 | 35.9±0.6 | (46.1±1.5)* | 44.0±1.7 | 45.9±0.8 |
C 18:2 | 36.6±0.9 | (25.1±1.7)* | 41.5±1.1 | (35.6±1.1)* |
C 18:3 | 1.8±0.1 | (1.3±0.3)* | 1.3±0.2 | 1.4±0.3 |
C 20:0 | 0.5±0.2 | 0.3±0.2 | n.d. | n.d. |
C 20:1 | 0.2±0.1 | 0.4±0.3 | 0.5±0.1 | 0.48±0.09 |
UFA | 76.9±0.9 | (74.6±0.7)* | 90.6±1.1 | (86.1±0.8)* |
mono- | 38.4±0.3 | (48.2±0.7)* | 47.8±1.3 | 49.1±1.6 |
poli- | 38.5±0.9 | (26.4±0.8)* | 42.8±1.00 | (37.0±2.1)* |
C16 | 16.5±0.8 | 15.4±0.3 | 10.4±0.9 | (14.1±1.1)* |
C18 | 82.4±0.8 | (83.7±0.5)* | (88.4±0.7) | (82.9±1.4)* |
SFA/UFA | 0.30±0.02 | (0.34±0.01)* | 0.10±0.01 | (0.17±0.01)* |
IU | 1.18±0.02 | (1.03±0.02)* | 1.36±0.02 | (1.25±0.01)* |
Mean value±S.D. of at least three independent experiments performed in duplicate was calculated on the basis of peak areas; the results represent the mean value; *significantly different from the wild type (Mann-Whitney U test, p<0.05)