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. 2020 Aug;20(3):372–380. doi: 10.17305/bjbms.2019.4410

FIGURE 4.

FIGURE 4

Phosphatase and tensin homolog deleted on chromosome ten (PTEN) was a target gene of miRNA-155-5p. (A) The binding sites were predicted on microRNA.org. (B) Dual luciferase activity was measured by dual luciferase reporter assay to confirm the relationship between PTEN and miRNA-155-5p. (C) MiR-155-5p and PTEN expression was detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in NRK-52E cells. (D) PTEN expression was detected by western blotting in NRK-52E cells. PTEN expression was upregulated with miRNA-155-5p suppression. **p < 0.01 compared with NC mimic group; #p < 0.05, ##p < 0.01 compared with NC inhibitor group. WT: Wild type; UTR: Untranslated region; NC: Negative control; MUT: Mutant.