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PUR activation of Shh on OGD-induced CREB and BDNF expressions: (a) protein levels of p-CREB, CREB, and BDNF at 4 h after OGD as determined with the use of Western blot (N = 3/group); (b) the level of BDNF mRNA at 4 h after OGD as determined with the use of qRT-PCR (N = 3/group). Values represent the mean ± SD, ∗p < 0.05, and ∗∗p < 0.01 according to ANOVA with Tukey's post hoc comparisons.
