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. 2020 Aug 7;205(4):994–1008. doi: 10.4049/jimmunol.2000372

FIGURE 1.

FIGURE 1.

Generation of il4/13a−/− and il4/13b−/− zebrafish mutants by CRISPR/Cas9. (A) Zebrafish il4/13a and il4/13b genes. (B–G) qPCR analysis showing the levels of il4/13a and il4/13b transcripts in wildtype, il4/13a−/−, il4/13b−/−, and il4/13a;b-/- 5 dpf larvae. Gene expression was normalized to the expression of β−actin. Error bars represent SEM; n > 3, *p < 0.05, **p < 0.01, ***p < 0.001. (H) Sequence of wildtype and mutated IL-4/13A and IL-4/13B proteins. Underlined is the unchanged portion of the protein sequence in mutant animals. Yellow-shaded “R” indicates the conserved arginine residue required in the human homologs for receptor binding. The asterisks indicate a stop codon.