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. 2020 Aug 7;205(4):994–1008. doi: 10.4049/jimmunol.2000372

FIGURE 4.

FIGURE 4.

Mutation of il10 induces morphological changes in the gills of adult zebrafish. (A) Representative images of sections (n = 3) of gills from wildtype, il4/13a−/−, il4/13b−/−, il4/13a;b−/−, and il10e46/e46 animals stained with H&E. Gill arch (A), filaments (F), and lamellae (L) are indicated. Lamellar epithelial cell alteration (asterisks), filament thickening (triangle), and arch thickening (arrow) are indicated. (B) Quantification of gill tissue damage in wildtype, il4/13a−/−, il4/13b−/−, il4/13a;b−/−, and il10e46/e46. Widespread alteration is observed in the gills of il10e46/e46 animals. (C) Bar graph showing significantly enriched ontologies in gills of il10e46/e46 animals. (D) Heatmap of NES for indicated gene sets generated by GSEA in gills of il10e46/e46 compared with wildtype animals. (E) qPCR analysis showing transcript levels of il1β, il6, tnfα, and ifng1–2 in gills of il10e46/e46 compared with wildtype animals. Gene expression was normalized to the expression of β−actin. Error bars represent SEM; n > 3, *p < 0.05.