FIGURE 6.
Prolonged inflammatory response in il10e46/e46 gills following R848 stimulation. (A) qPCR analysis showing transcript levels of il1β, il6, tnfα, and ifng1–2 in gills from wildtype and il10e46/e46 animals following R848 challenge for 1 and 8 h. Gene expression was normalized to the expression of β−actin. Fold change is shown. Error bars represent SEM; n > 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) Representative images of sections (n = 3) of gills from wildtype and il10e46/e46 animals (n = 3) stained with H&E following 8-h R848 challenge. Epithelial cell alteration in the lamellae (black arrows) and gill filament (stars) are indicated in gills from il10e46/e46 animals. Scale bar, 50 μm. (C) Quantification of gill tissue damage in wildtype and il10e46/e46 animals following R848 challenge.