FIGURE 3.

Activated Vδ2⁺ cells promote enhanced maturation of S. aureus–infected DCs. DCs (5 × 10⁵/ml) were infected with S. aureus (strains PS80, USA300, and Newman) or E. coli (strain CFT073) at multiplicity of infection 10 for 3 h before the elimination of extracellular bacteria by gentamicin treatment. DCs were then cultured alone or with γδ T cells (5 × 10⁵/ml) for 24 h. IL-12p70 (A) and TNF (B) levels in culture supernatants at 20 h were assessed by ELISA. Results are expressed as mean concentration in culture supernatants ± SEM. n = 3–14 DC donors per group. n = 6–28 γδ T cell donors per group. Statistical analysis by pairwise Wilcoxon signed-rank test. Cells were treated with BFA for a further 4 h before analysis by flow cytometry. Results are expressed as mean IL-12p40⁺ cells within total live singlet cells of each subset ± SEM (C). Representative FACS plot for PS80 infection is shown (D). Expression of CD86 (E) and CCR7 (F) was also assessed and expressed as the mean fluorescence intensity of total live singlet DC-SIGN⁺ cells ± SEM. n = 8–12 DC donors per group. n = 8–12 γδ T cells donors per group. Statistical analysis by pairwise Wilcoxon signed-rank test. *p < 0.05, ***p < 0.001.