FIGURE 4.

Activated Vδ2⁺ cells promote enhanced maturation of S. aureus–infected DCs through IFN-γ secretion and direct contact. DCs (5 × 10⁵/ml) were infected with S. aureus (strain PS80) at multiplicity of infection 10 for 3 h before the elimination of extracellular bacteria by gentamicin treatment. DCs were then cultured alone or with γδ T cells (5 × 10⁵/ml) for 24 h, either in a membrane-separated coculture (A and D) or treated with anti–IFN-γ (B and E) or anti-γδTCR (C) Abs. IL-12p70 levels in culture supernatants at 20 h was assessed by ELISA (A–C). Results are expressed as mean IL-12p70 concentration in culture supernatants ± SEM. Expression of CD86 was assessed by flow cytometry (D and E). Results are expressed as the mean fluorescence intensity of total live singlet DC-SIGN⁺ cells ± SEM. n = 7–15 DC donors per group. n = 7–15 γδ T cell donors per group. Statistical analysis by pairwise Wilcoxon signed-rank test. *p < 0.05, **p < 0.01, ***p < 0.001.