Skip to main content
. Author manuscript; available in PMC: 2021 Aug 3.
Published in final edited form as: Curr Biol. 2020 Jun 11;30(15):3016–3023.e3. doi: 10.1016/j.cub.2020.05.056

Figure 4. Microtubule growth rates as a function of local microtubule plus-end density.

Figure 4.

Pictorial representation (A) of local density analysis, showing a series of search radii projected from the center of an EB1 comet over its lifetime (scale bar = 5 μm). (B) A micro-enclosure supplemented with two aMTOCs depicted as a max-intensity z-projection (left panel). The average velocity and average local density of each EB1 comet plotted at each coordinate position of the comet’s lifetime (center and right panel, respectively). The average local density indicates the number of EB1 comets detected within a 3 μm search radius. See also Figure S3. (C) Average local density as a function of the search radius (indicated by blue circles), displayed as a box plot with bin sizes being equal to a 10% increment of the maximum density observed at that search radius (red dashed line indicates global density of the micro-enclosure). Box plots feature a Tukey-style IQR, with whiskers indicating one SD of the median. Notches approximate the 95% confidence interval of the median. See also Figure S2D and S4. See also Video S4.