Fig 6.

Eosinophils are the major source of IL-4 within ANAs during early regeneration. IL-4-GFP mice sciatic nerve were repaired using ANA to identify sources of IL-4. A) Representative images and quantification of T cells (CD3, red) and IL4-GFP cells (green) within ANAs at 10, 14, and 18 days after ANA repair. P values across time for each group are indicated in a matching color. B) Representative images and quantification of T cells and eosinophils (Siglec F) colocalization with GFP expressing cells at 14 days. C) Representative flow cytometry and quantification of proportion of neutrophils, macrophages, eosinophils, and T cells from spleen expressing GFP at 14 days after ANA repair. Neutrophils were gated on CD45+CD11b+Ly6G+. Macrophages were gated on CD45⁺CD11b⁺F4/80⁺CD64⁺. Eosinophils were gated on CD45⁺CD11b⁺SiglecF⁺. T cells were gated on CD45⁺CD11b⁺CD3⁺. Mean ± SD, n=3/group; p values shown. Scale bar represent 50 μm.