CD4 T cells and IL-4 are critical to early myelination of axons within ANAs. WT mice sciatic nerve were repaired using ANA and treated with antibodies to deplete CD4 T cells or neutralize IL-4 up to the 14 day endpoint. A&B) Representative immunofluorescence and quantification of myelin (MBP, red), neurofilament (NF200, green), and Schwann cells (S100, green) within ANAs for CD4 depletion groups. C&D) Representative immunofluorescence and quantification of myelin (MBP, red), neurofilament (NF200, green), and Schwann cells (S100, green) within ANAs for IL-4 neutralization groups. Mean ± SD, n=5/group; p values shown. Scale bar represent 50 μm.