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. 2020 Aug 3;10:1164. doi: 10.3389/fonc.2020.01164

Figure 2.

Figure 2

ERK5-ko suppresses tumor growth kinetics and metastasis of MBA-MB-231 cells in vivo. (A) Female SCID/beige mice (n = 5/group) were bilaterally injected into the MFP with MDA-MB-231 vector parental and -ERK5-ko cells. Data points represent mean tumor volume ± SEM. (B) At day 28 post-cell injection, primary tumors were excised and weighed; this facilitated distant metastatic seeding. While 10 injections were performed (two injections of cancer cells per mouse), only eight tumors formed in the MDA-MB-231 control group, and nine tumors formed in the MDA-MB-231-ERK5-ko group. (C) Total area of metastases was quantified using Aperio ImageScope software (Leica Biosystems). Points represent total area of metastases per lung section ± SEM. Parental, n = 3; ERK5-ko, n = 4. (D) Number of metastatic lesions was determined. *p < 0.05, ***p < 0.001. (E) H & E staining of lungs 30 days post-survival surgery was used to visualize metastases. Representative lungs from each group demonstrate decreased metastasis in the ERK5 knockout injected animals. Black arrows depict examples of metastases in the lung tissues. Magnified regions were viewed at 200×.