Figure 3.

ERK5 deletion results in loss of extracellular matrix components. RNA-sequencing was performed with the MDA-MB-231- and Hs-578T-parental and -ERK5-ko cell lines. Genes were identified that were commonly (A) upregulated and (B) downregulated in TNBC ERK5-ko cells normalized to parental controls. (C) Pathway analysis of the RNA-sequencing showed significant downregulation of signaling pathways that organize the extracellular matrix, regulation of cell growth and regulation of cell adhesion. (D) qRT-PCR validation of ECM-associated genes identified from RNA-sequencing. Data was normalized to β-actin and parental controls. Data is represented as averages ± SEM of triplicate experiments. *p < 0.05, **p < 0.01, ***p < 0.001.