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. Author manuscript; available in PMC: 2020 Nov 8.
Published in final edited form as: J Mol Biol. 2019 Aug 30;431(22):4455–4474. doi: 10.1016/j.jmb.2019.08.012

Figure 3. In vitro cos cleavage activity of terminases.

Figure 3.

Cleavage assay results for selected mutants. Cleavage of the 5.1 kb substrate DNA at cos produces 2.9 and 2.2 kb product DNAs. A. Standard reactions done at 30°C. B. Reactions done at 22°C and 42°C with the cold-sensitive gpA D178E terminase. Unlabeled lanes are 1 kb ladder (New England Biolabs). The A175L extracts contained an EDTA-free protease inhibitor cocktail (“cOmplete Mini”, Roche, Inc.) at the recommended concentration.