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The data presented in Figure 5B were fit to a monophasic reaction time course and a three-state kinetic model as described in Materials and Methods. The derived rate constants are presented with standard deviations as indicated.
| Enzyme | Monophasic Model | Three-State Model | |
|---|---|---|---|
| kobs (s−1) | k1 (M−1s−1) | k2 (s−1) | |
| WT | (0.139 ± 0.016) | - | - |
| D178N | (1.0 ± 0.4) x 10−3 | (1.7 ± 0.2) x 104 | (2.4 ± 0.1) x 10−3 |
| R79A | - | (1.3 ± 0.9) x 104 | (5.3 ± 2.9) x 10−3 |
