Figure 6.

Functional analysis of predicted metagenomes. (A) Differentially abundant KEGG pathways across AIH (N = 37) and HCs (N = 78) identified by LEfSe. Histogram of LDA scores calculated for selected pathways showing significant difference in gene functions between AIH (green) and HCs (red). LDA score on log₁₀ scale indicated at bottom. Significance of the microbial marker increases with LDA score. AIH, autoimmune hepatitis; HCs, healthy controls; OTUs, operational taxonomic units; LEfSe, linear discriminant analysis effect size; LDA, linear discriminant analysis. (B) Scatterplots of relative abundances of histidine metabolism and age. A positive correlation was found between the abundance of histidine metabolism and age (Spearman's correlation coefficient = 4.48e−04, FDR = 0.2389, N = 115). AIH, autoimmune hepatitis; LDA, linear discriminant analysis; LEfSe, linear discriminant analysis effect size; MaAsLin, multivariate association with linear models algorithm.