Table 1.

Kinetic parameters of Hv and mHv proteins binding with receptors and mutants from SPRi

No.	analytes	ligands	Avg ka	Avg kd	Avg KD	Interactions	ABS (tr_KD)
			(1/Ms)	(1/s)	(M)	intensity
1	SLAM	Hv	8.88E+02	9.59E-04	1.08E-06	Strong	19.8205
2	Nectin 4	Hv	1.42E+03	4.35E-04	3.06E-07	Strong	21.6395
3	mSLAM1	Hv	1.78E+01	5.06E-02	2.85E-03	Weak	8.4558
4	mSLAM2	Hv	8.45E+01	4.41E-03	5.22E-05	Middle	14.2250
5	mSLAM3	Hv	7.21E+00	2.34E-01	3.25E-02	VW/None	4.9432
6	mNectin 4	Hv	6.21E+02	1.11E-03	1.79E-06	Strong	19.0881
7	Hv	Hv	1.81E+01	5.14E-02	2.83E-03	Weak	8.4626
8	PBS	Hv	1.30E+00	2.80E-01	2.16E-01	VW/None	2.2111
9	SLAM	mHv	4.29E+00	1.33E-01	3.09E-02	VW/None	5.0177
10	Nectin 4	mHv	9.85E+01	2.08E-03	2.11E-05	Middle	15.5312
11	mSLAM1	mHv	5.64E+00	1.58E-01	2.80E-02	VW/None	5.1585
12	mSLAM2	mHv	1.37E+01	3.52E-01	2.56E-02	VW/None	5.2851
13	mSLAM3	mHv	2.99E+00	3.29E-01	1.10E-01	VW/None	3.1839
14	mNectin 4	mHv	1.81E+01	7.21E-02	3.99E-03	Weak	7.9697
15	mHv	mHv	1.87E+01	4.61E-02	2.47E-03	Weak	8.6604
16	PBS	mHv	1.95E+00	1.82E-01	9.33E-02	VW/None	3.4220

Interaction intensity level: 10⁻¹³–10⁻⁸, very strong; 10⁻⁸–10⁻⁵,strong; 10⁻⁵–10⁻³, medium; 10⁻³–2×10⁻²,weak; 2×10⁻²–10², very week (VW)/ none).

Avg k _a, average association rate constant; Avg k _d, average dissociation rate constant; Avg K _D, average equilibrium dissociation constant (k _d /k _a); ABS (tr_ K _D), absolute affinity coefficient [log₂(K _D)].