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. Author manuscript; available in PMC: 2020 Aug 10.
Published in final edited form as: Cancer Res. 2020 Jan 16;80(7):1524–1537. doi: 10.1158/0008-5472.CAN-19-2054

Figure 1. ABCC4 is highly expressed in SHH-MB.

Figure 1.

(A) Abcc4 expression in MB cohort from GSE37385, n= 1087 (Northcott et al., 2012b). * P < 0.05, ** P < 0.01, **** P < 0.0001, one-way ANOVA.

(B) Data from (A) where ABCC4 expression and SHH signaling genes are compared in a heatmap.

(C) SHH-MB patients with available survival and gene expression information from GSE85217, n = 763 (Cavalli et al., 2017). The relationship between ABCC4 expression and survival time was analyzed using the Kaplan-Meier method with log-rank statistics.

(D) Analysis of correlation between GLI2 and ABCC4 expression levels from GSE85217 data, n =763. Spearman’s correlation r was 0.6182, P<0.0001.

(E) Enrichment of predicted GLI1, GLI2, GLI3 transcriptional regulons and ABCC4 signaling regulon in differentially expressed genes between SHH and other MB subgroups from GSE85217, n - 763 (Cavalli et al., 2017). P values of regulon enrichment and differential expression of the hub gene are indicated in the DA DE table.

F) Wild type mouse brains were fixed at indicated post-natal days (P0, P3, P7, and P10) and immunostained with antibodies to mouse ABCC4 (anti-ABCC4). Scale bars = 5 mm and 50 μm

(G) Sagittal sections of SHH-MB mouse models (Ptch+/−;Cdkn2c−/−, and Ptch+/−;Trp53−/−) immunostained with anti-ABCC4. Scale bar = 5 mm and 25 μm.

(H) Sagittal sections of SHH-MB patient-derived xenografts. Sections were stained with H&E and with anti-KI67 and anti-ABCC4. Scale bar = 5 mm and 200 μm.