4.1. Table 1.
Reagents used in this study
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-V5 tag mouse monoclonal antibody | Thermo Fisher Scientific, USA | CAT#: R960-25 |
| Anti-V5 tag antibody (agarose) | Abcam, USA | CAT#: Ab1229 |
| Rabbit polyclonal anti-dP75 | This study | N/A |
| Chicken anti-Jil-1 | Johansen lab | N/A |
| Mouse monoclonal anti-H3K9me2-ChIP grade | Abcam | CAT#: Ab1220 |
| Mouse monoclonal anti-Flag M2 antibody | Sigma chemical, USA | CAT#: F3165-.2MG |
| Anti-alpha-tubulin antibody | DSHB, USA | 12G10 |
| Alexa 488 Donkey anti-Mouse | Thermo Fisher Scientific | CAT#: A-21202 |
| Alexa 568 Goat anti-Mouse | Thermo Fisher Scientific | CAT#: A-11004 |
| Alexa 488 Goat anti-Chicken | Thermo Fisher Scientific | CAT#: A-11039 |
| Alexa 568 Goat anti-Rabbit | Thermo Fisher Scientific | CAT#: A-11011 |
| Amersham ECL Mouse IgG, HRP-linked whole Ab | GE Healthcare Life Science, USA | NXA931-1ML |
| Amersham ECL Rabbit IgG, HRP-linked whole Ab | GE Healthcare Life Science | NA934-1ML |
| Experimental Models: Organisms/Strains | ||
| D.melanogaster: w; Act5c-Gal4/CyO | Zhao Zhang lab | N/A |
| D.melanogaster: P(otu-Gal4::VP16.R)1 w[*]; P(Gal4-nos.NGT)40; P(Gal4::VP16nos.UTR)CG6325[MVD1] | Bloomington Drosophila Stock Center, USA | CAT#: 31777 |
| D.melanogaster: jil-1-sh (attP2) | Bloomington Drosophila Stock Center | 57293 |
| D.melanogaster: mcherry-sh (attP2) | Bloomington Drosophila Stock Center | 35785 |
| D.melanogaster: dP75-sh (attP40) | Bloomington Drosophila Stock Center | 55274 |
| D.melanogaster: V5::dP75 (at its endogenous locus) | This study | N/A |
| D.melanogaster: UASP-FlagMyc-dP75 (attP40) | This study | N/A |
| D.melanogaster: UASP-FlagMyc-Jil-1 (attP2) | This study | N/A |
| D.melanogaster: dP75-sg1/TM6c, sb | This study | N/A |
| D.melanogaster: dP75-sg2/TM6c, sb | This study | N/A |
| Competent cells | ||
| Top10 one shot kit | Life Technologies, USA | C404006 |
| BL21 (DE3) | NEB, USA | C2527H |
| Critical Commercial Assay | ||
| Vectashield Mounting Medium | VECTOR LABORATORIES INC MS | CAT#: 101098-042 |
| V5 peptide | abcam | Ab15829 |
| EDTA free complete protease inhibitor | Roche, USA | CAT#: 4693159001 |
| pENTR™/D-TOPO® Cloning Kit | Thermo Fisher Scientific | K240020 |
| Gateway™ LR Clonase™ II Enzyme mix | Thermo Fisher Scientific | 11791020 |
| Deposited Data | ||
| Illumina Sequencing raw data (FASTQ) | This study | |
| Oligonucleotides | ||
| Forward primer to validate V5 knockin: ACC CTC TTC TTG GTC TAG ATA | This study | N/A |
| Reverse primer to validate V5 knockin: AGC ATC TCG TTC CGG ATG TT | This study | N/A |
| Forward primer to sequence V5 knockin fly: ATA CGA GCA CTG CTA GCG C | This study | N/A |
| Forward primer to validate dP75 knockout: ACC AAA CGA GCT AGA AGT CAA | This study | N/A |
| Reverse primer to validate dP75 knockout: ATC GAT GGC CTC GAT GAA CT | This study | N/A |
| Forward primer to generate dP75 transgene: CAC CAT GGG TAA GGA AAA GGC GGC | This study | N/A |
| Reverse primer to generate dP75 transgene: TTA CTG TGG AGC CGA TCC G | This study | N/A |
| Forward primer to generate jil-1 transgene: CAC CAT GAG TCG CTT GCA AAA GCA A | This study | N/A |
| Reverse primer to generate jil-1 transgene: TCA TTG GAA CTG ATA AAG TTG AC | This study | N/A |
| Software and Algorithms | ||
| piPipes | Han et al. (2015) | N/A |