Fig. 1.
Design of FP probe II138. A. Methylation of nicotinamide (NAM) by NNMT. B. Structure of the fluorescent probe II138 comprised of LL320 (black), the linker (red), and fluorescein (green). C. Crystal structure of hNNMT-LL320 complex (PDB: 6PVS). hNNMT is colored gray and LL320 is shown in green stick with N6 adenosine pointing towards the solvent. B. The distance between N6 and the protein surface is 4.2 Å.