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. 2020 Jun 3;1(1):100011. doi: 10.1016/j.xpro.2019.100011

Figure 5.

Figure 5

Typical elution profiles from each chromatography step

(A and B) Elution profile of recombinant human α1B/β3-tubulin from two 1 mL HisTrap columns (A) and two 1 mL StrepTrap columns (B).

(C) The chromatogram of the flow-through from HiTrap SP/HisTrap HP columns.

(D and E) Elution profile of recombinant human α1B/β3-tubulin (D; peak volume, 81.1 mL) or bovine brain tubulin (E; peak volume, 80.4 mL) from size-exclusion chromatography. *, non-specific instrument singal. V0, void volume. 1CV, one column volume.