Lifelong suppression of gut microflora prevents inflammation and autoimmunity in C9orf72 LOF mice
a, Male and female C9orf72Harvard +/+ and −/− neo deleted mice of weaning age were cohoused by treatment group, then administered vehicle (+/+ n=7; −/− n=11) or antibiotics (+/+ n=7; −/− n=11) daily for life. Mice assessed for gut microbial composition (b, 4 weeks), blood measures (c-e, 8 weeks) and sacrificed for organ and CNS assessment (f, 28 weeks). b, 16S rDNA sequencing of bacteria diversity in feces. Each dot represents total sequencing reads per cage. One-way ANOVA with Dunnett multiple comparison. c, Blood neutrophil count. d, Blood platelet count measured at 0ºC. e, Plasma anti-dsDNA antibody activity. f, Spleen weight. c-f, One way ANOVA with Sidak multiple comparisons. Each dot represents one animal.