Fig 4. EGL-4 and CNG-2 work concurrently in parallel pathways downstream of cGMP to induce daf-7.

(A) Epistasis analysis using cng-2(loss-of-function) and egl-4(gain-of-function) alleles. Animals were maintained on the E. coli strain OP50. (B-C) Maximum fluorescence values of Pdaf-7::gfp in ASJ neurons of various mutants following exposure to 5mM pCPT-cGMP; animals were maintained on the E. coli strain OP50. All error bars indicate standard deviation. ****p < 0.0001 by Mann-Whitney U test.