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. 2020 Aug 10;15(8):e0236781. doi: 10.1371/journal.pone.0236781

Fig 7. Bach1 regulates Mhc and MRFs mRNA expression.

Fig 7

(A) Immunohistochemical staining of Bach1 and nuclei (Hoechst) of C2C12 cells 6 days after inducing differentiation. (B, C) Expression levels of Myh7 (MHC Ⅰ/b) and Myh1 (MHC Ⅱd/x)mRNA (B) and Myogenic regulatory factors (MRFs: Myod1 (MyoD), Myf5, Myog (myogenin) and Myf6 (Mrf4))mRNA (C) measured by RT-qPCR of Bach1-silenced and control C2C12 cells before (day 0) and after inducing differentiation (day 2, 4 and 6). (n = 3; * p< 0.05, ** p< 0.01, *** p < 0.001).