Table 1.

Experimental Procedures

Experimental Days		Dep. State*	Solutions
1-min Tests		Experiment 1: TRPM5 KO, T1R3 KO, WT Groups
1 – 2	WR Two-Bottle Test	Water restricted	8% glucose vs. 8% fructose
3	FR Two-Bottle Test	Food restricted	8% glucose vs. 8% fructose
1-h Tests
4 – 5	Two-Bottle Test 1	Food restricted	8% glucose vs. 8% fructose
6 – 9	One-Bottle Train	Food restricted	8% glucose and 8% fructose
10 – 11	Two-Bottle Test 2	Food restricted	8% glucose vs. 8% fructose
24-h Home Cage Tests
12 – 15	One-Bottle Train	Food restricted	TRPM5 KO, WT: 8% glucose and 8% fructose
16 – 17	Two-Bottle Test	Food restricted	TRPM5 KO, WT: 8% glucose vs. 8% fructose
12 – 13	Two-Bottle Test	Food ad lib	T1R3 KO Lo, Hi: 0.2% saccharin vs. water
1-min Tests		Experiment 2: T1R3 KO, WT Groups
1 – 2	WR Two-Bottle Test	Water restricted	8% glucose vs. 8% fructose
3	FR Two-Bottle Test	Food restricted	8% glucose vs. 8% fructose
1-h Tests
4 – 5	Two-Bottle Test 1	Food restricted	8% glucose vs. 8% fructose
6 – 9	One-Bottle Train	Food restricted	8% glucose and 8% fructose
10 – 11	Two-Bottle Test 2	Food restricted	8% glucose vs. 8% fructose
13 – 14	Two-Bottle Test 3	Food restricted	0.2% saccharin vs. water
24-h Home Cage Tests
15 – 16	Two-Bottle Test	Food ad lib	8% glucose vs. 8% fructose
17 – 18	Two-Bottle Test	Food ad lib	8% fructose vs. water
1-min Tests		Experiment 3: T1R3 KO, WT Groups
1 – 2	WR Two-Bottle Test	Water restricted	8% glucose vs. 8.6% MDG
3	FR Two-Bottle Test	Food restricted	8% glucose vs. 8.6% MDG
1-h Tests
4 – 5	Two-Bottle Test 1	Food restricted	8% glucose vs. 8.6% MDG
6 – 9	One-Bottle Train	Food restricted	8% glucose and 8.6% MDG
10 – 11	Two-Bottle Test 2	Food restricted	8% glucose vs. 8.6% MDG
13 – 14	Two-Bottle Test 3	Food restricted	0.2% saccharin vs. water

^*Deprivation states described in Methods. All 1-min and 1-h tests conducted in lick test chambers; 24-h tests conducted in home cages.