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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Immunol. 2020 Jun 26;205(3):630–636. doi: 10.4049/jimmunol.2000101

Figure 4. N-glycan branching limits immature B cell negative selection by limiting BCR engagement.

Figure 4.

(a) Flow cytometric analysis of ex vivo B220+IgM+ bone marrow B cells for intracellular staining of Nur77. Each dot represents one mouse of the indicated genotypes; unpaired one-tailed Mann-Whitney t-test. *P = 0.05. (b-c) Annexin V binding to bone marrow B cells following a 1-day culture with high doses of anti-IgM F(ab’)2, gated on B220+IgM= (b) and B220+IgM+ (c) cells. n = 3. Kruskal-Wallis test with false discovery rate correction (Benjamini, Krieger and Yekutieli method) for multiple comparisons, with comparison to zero anti-IgM for each genotype. Error bars indicate mean ± s.e.m. *P < 0.05.