Figure 1.

Expression of XCL1 and XCR1 in periprosthetic tissues. (A–C) Detection of XCR1 in human tissues around loosening hip-implant by the immunofluorescence test. Deparaffinized sections were stained to observe F4/80, CD68, iNOS (green), XCR1 (red), and cell nuclei (blue). Scale bars are 100 μm. Representative images exhibit the sectioned tissues from three patients. (D) Detection of XCL1 in synovial fluid from same patients by Western blotting analysis. (E) Gene expressions of XCL1 and XCR 1 in calvarial bone tissues in a murine osteolysis calvarial model. Results represent the means of relative expression values ± SEM of three mice. ^*indicates a significant difference as determined by the Student t-test (p ≤ 0.05).