Figure 3.

Blockade of XCL1/lymphotactin by neutralizing antibody ameliorates severity of osteolysis triggered by polyethylene-particles in murine model. (A) Quantification of the lytic area on the calvarial bone tissues of mice determined by micro-CT. Left panel shows representative images of calvariae. Results represent the means ± SEM of seven mice. ^*represents the significance determined by one-way ANOVA, followed by a Tukey's multiple-comparison procedure. Arrows indicate osteolytic lesions. (B) Histological analyses of bone sections stained by H&E and TRAP. Scale bar is 100 μm. (C) Cell count of inflammatory cells in calvarial bone sections. (D) Quantification of TRAP-stained areas in calvarial bone sections. Arrows indicate bone lesions. The results represent the means ± SEM for four mice. ^*indicates a significant difference, as determined the Student t-test (p ≤ 0.05).