Figure 1.

Cloning and expression of fragments spanning the full-length of Factor H. The illustrated recombinant proteins were generated using primers that begin and end in the inter-domain spacers and an expression vector designed specifically to express CCP domains as briefly described in Materials and methods. Full length Factor H (blue), and all of the Factor H sub-fragments depicted above it, were expressed in insect cells, whereas the three-domain fragments depicted below full length Factor H were expressed in the yeast system. Three constructs (shown in red) did not express in the yeast (Pichia) system and one expressed poorly (yellow). Nevertheless, as shown, the entire length was covered so that each domain was expressed in at least twice in the resulting family of proteins.