Figure 7.

Relative affinity of three-domain Factor H fragments for heparin. Recombinant three-domain fragments of Factor H were injected over a sensor chip coated with heparin which was attached through a single amino group incorporated at the reducing end of the polysaccharide chain. This yields heparin with a single orientation and with all sulfate groups intact. The SPR measurements shown are the result of injecting each fragment at a concentration of 3 μM in HBS-P over the heparin-coated surface. Individual sensorgrams for each three-domain fragment are shown in Supplemental Figure 1.