Figure 5.

oprC deficiency decreased pyroptosis and STAT3/ NF-κB phosphorylation. (A) MH-S cells challenged with bacteria at a multiplicity of infection (MOI) of 10 for 2 h. Immunoblotting analysis of GSDMD, NF-κB/p65, p-NF-κB/p65, STAT3, and p-STAT3. (B,C) Secreted IL-1β and LDH from the supernatant was assessed by ELISA and LDH assay kit after MH-S cells were infected at an MOI of 10 for 2 h (n = 3). (D) Representative images of immunofluorescence staining of the lungs infected with bacteria for p-STAT3 co-stained with p-NF-κB/p65 and DAPI (n = 3). Arrows indicate the colocalizations between p-STAT3 and p-NF-κB/p65. Scale bars, 10 μm. Error bars represent the mean ± s.d. One-way ANOVA with a post-hoc Tukey test was performed for comparison of means of groups.