Fig. 4.

miR-486-3p overexpression increases chemosensitivity to temozolomide. a Cell proliferation was evaluated in U251-TR and A172 cells stably expressing miR-NC or miR-486-3p with the TMZ treatments at different concentration. CCK8 assay was performed 48 h after treatment. b Cell proliferation in 200 μM TMZ treatments was tested every 24 h in U251-TR and A172 cells overexpressing miR-486-3p and miR-NC. c Western blot analysis of MGMT, cleaved caspase-3, and γ-H2AX expression in U251-TR cells co-transfected with miR-486-3p or miR-NC, and cultured in 200 μM TMZ. The data represent results from one of three independent experiments. *P < 0.05, **P < 0.01