Fig. 5.

Allicin promotes high expression of miR-486-3p that enhances TMZ sensitivity in tumor-bearing mice. a Overexpression miR-486-3p reduced the U251-RT cells viability. Data presented as mean ± SEM of cell viability from three independent experiments with triplicates in each experiment. b Western blot analysis confirmed that after 48 h treatment, miR-486-3p-overexpression group and miR-NC group had a significant decrease in MGMT expression.c Expression analysis of cleaved caspase-3 showed that miR-486-3p overexpression group and miR-NC group promote apoptosis significantly. The data represent results from one of three independent experiments. d Survival curve of miR-486-3p overexpression, miR-486-3p inhibitor, and miR-NC control groups. *P < 0.05, **P < 0.01 versus miR-486-3p inhibitor group