Figure 2.

Effect of glucose on miR-1281 and VEGFA expression. (A,B) ARPE-19 epithelial cells and HUVEC endothelial cells were exposed to low (5 mM) or high (25 mM) glucose, and both extracellular (white bar) and intracellular (gray bar) levels of miR-1281 were obtained after 48 h incubation. Results are the mean ± SEM of three independent experiments, each in triplicate. *p < 0.05; **p < 0.001. (C) VEGFA mRNA and protein expression in ARPE-19 and HUVEC cells, as measured by RT-qPCR (bar graphs) and western blot (WB), respectively. mRNA levels are shown as means ± SEM of three independent experiments, each performed in triplicate. *p < 0.05. Representative WBs of VEGFA protein in both cell types are representative of at least 3 independent experiments. β-Actin, control of protein loading.