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. 2020 Aug 4;8:760. doi: 10.3389/fbioe.2020.00760

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Copyright © 2020 Schmidt, Markus, Kandarova and Wiest.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Setup for the two fluidic lines. A TEER measurement liquid or a test substance can be applied via the apical line. Valves 2 and 3 regulate the ALI. For the basolateral line, valve 4 is used to provide new nutrients to the BioChip. Valves 5 and 6 are switched to filtered air when the medium in the other line is pumped (MM, maintenance medium; TS, test substance; LBM, low buffered medium; V.1, valve #1; PMP.2, pump channel #2; BD.1, air bubble detector #1; NC, normally closed; NO, normally open).