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. 2020 Aug 4;11:1724. doi: 10.3389/fimmu.2020.01724

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Copyright © 2020 Al Heialy, Gaudet, Ramakrishnan, Mogas, Salameh, Mahboub and Hamid.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stimulation with IL-17A&F and IL-17A alone induces changes in inflammatory cytokine profiles in adipocytes from lean and obese subjects. A multiplex assay was used to measure the levels of inflammatory cytokines: IL-8 (A), IL-6 (B), IL-5 (C), IL-4 (D), and IFN-γ (E) secreted by mature adipocytes after 48 h stimulation with IL-17A & F combination or IL-17A alone. One independent experiment preformed per subject. n = 4 lean subjects, n = 3 obese subjects, One-way ANOVA, Mean ± SE; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.