FIGURE 3.

The lipid composition of S. cerevisiae WT indifferent media. A single colony of the BY4741 WT strain was used to inoculate a preculture in either synthetic (SCD) or rich (YPD) medium. After overnight cultivation for 21 h, a fresh culture was inoculated to an OD₆₀₀ of 0.1. When the cells reached an OD₆₀₀ of 0.80 ± 0.05, they were cultivated for one more hour. 20 OD equivalents of these cells were harvested and analyzed by lipid mass spectrometry. The data represented by black and white bars relate to cells cultivated in rich and synthetic medium, respectively [except for panels (F and G)]. (A) Lipid class composition in mol% of all quantified lipids in the sample organized by the categories: sterol (orange), sphingolipids (magenta), membrane glycerolipids (green, classes with two acyl chains highlighted by green box), and storage lipids (blue). Erg, ergosterol; Cer, ceramide; IPC, inositolphosphorylceramide; MIPC, mannosy-IPC; M(IP)2C, mannosyl-di-IPC; CL, cardiolipin; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; DAG, diacylglycerol; LPC, lyso-PC; LPE, lyso-PE; LPI, lyso-PI; LPS, lyso-PS; TAG, triacylglycerol; EE, ergosteryl ester. (B) Profile of PA lipids in mol% of the class. (C) Total length of lipids in a sub-category of membrane glycerolipids (MGLs) including PA, PC, PE, PI, PS, DAG. The total length is given as the sum of carbon atoms in both fatty acyl chains in mol% of this sub-category. (D) Total number double bonds in a sub-category of MGLs (PA, PC, PE, PI, PS, DAG) is given as the sum of double bonds in both acyl chains, in mol% of this sub-category. (E) The acyl chain composition of PA, PC, PI and of a s sub-category of MGLs (PA, PC, PE, PI, PS, DAG) is normalized either to the individual lipid class or the sub-category and given in mol%. (F,G) The pairing of fatty acyl chains in MGLs (PA, PC, PE, PI, PS, DAG) is plotted for cells cultivated in (F) rich (YPD) and (G) synthetic (SCD) medium. The left panel indicates the pairing of fatty acyl chains in a sub-category of MGLs (PA, PC, PE, PI, PS, DAG) normalized to each particular fatty acyl chain and is given as mol%. The right panel indicates the abundance of acyl chain pairs in the sub-category of MGLs and is given in mol%. (H) Profile of sphingolipids in mol% of the category. The least abundant species in each panel are omitted for clarity. Each bar represents the average ± SD from n = 3 independent experiments. Statistical significance was tested by an unpaired two-tailed t-test using GraphPad Prism, *p < 0.05, **p < 0.01, ***p < 0.001.